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PW1 gene/paternally expressed gene 3 (PW1/Peg3) identifies multiple adult stem and progenitor cell populations


Details
Publication Date: 
12/07/2011
Authors: 
Besson V, Smeriglio P, Wegener A, Relaix F, Nait Oumesmar B, Sassoon DA, Marazzi G.
Citation: 
Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11470-5. Epub 2011 Jun 27.
DOI: 
10.1073/pnas.1103873108
Abstract
A variety of markers are invaluable for identifying and purifying stem/progenitor
      cells. Here we report the generation of a murine reporter line driven by Pw1 that
      reveals cycling and quiescent progenitor/stem cells in all adult tissues thus far
      examined, including the intestine, blood, testis, central nervous system, bone,
      skeletal muscle, and skin. Neurospheres generated from the adult PW1-reporter
      mouse show near 100% reporter-gene expression following a single passage.
      Furthermore, epidermal stem cells can be purified solely on the basis of
      reporter-gene expression. These cells are clonogenic, repopulate the epidermal
      stem-cell niches, and give rise to new hair follicles. Finally, we demonstrate
      that only PW1 reporter-expressing epidermal cells give rise to follicles that are
      capable of self-renewal following injury. Our data demonstrate that PW1 serves as
      an invaluable marker for competent self-renewing stem cells in a wide array of
      adult tissues, and the PW1-reporter mouse serves as a tool for rapid stem cell
      isolation and characterization.